Friday, October 25, 2013
Chemically Defined Medium as a Set Standard for Growth of Leuconostoc
Leuconostoc is a non-pathogenic, aero-tolerant anaerobic bacterial genus that lives on vegetation.
To accomplish the research goal, Megan will cultivate Leuconostoc species for 12 hours in a defined nutrient source and a complex nutrient source. Bacterial growth in each nutrient source will be determined through two methods called viable plate count (quantitative) and spectrophotometry (qualitative). Growth rates (m h-1) and bacterial colonies formed per milliliter of culture broth (CFU/ml) will be calculated from the quantitative viable plate count. Spectrophotometry will be used as a real-time growth assessment to monitor the progress of each growth experiment. Data indicating that there is no statistical difference in growth between each nutrient source would support the use of defined as a suitable alternative to complex. In addition, data indicating an increase in growth for the defined would also support its use as an alternative to complex. Megan's research advisor is Dr. Scott Holt.